Assayed definition

Thus, the intrinsic dynamic of neurospheres, as conventionally assayed, introduces confounders.

assay medical definition

Enzyme-linked immunoassay or EIA, enzyme linked immunosorbent assay. But some of the manipulations may be inseparable part of the assay itself and will not thus be considered pre-analytic.

This might have translated later possibly after the 14th century into a generalized meaning of analysis,[ citation needed ] e.

General steps[ edit ] An assay analysis is never an isolated process and must be combined with pre- and post-analytic procedures.

For assay of currency coinsthis literally meant analysis of the purity of the gold or silver or whatever precious component was used to represent the true value of the coin. Using time-lapse video microscopy, coculture experiments with genetically labeled cells, and analysis of the volume of spheres, we observed that neurospheres are highly motile structures prone to fuse even under ostensibly 'clonal' culture conditions.

Essayed meaning

We asked whether neurospheres are clonal structures whose diameter, number and composition accurately reflect the proliferation, self-renewal and multipotency of a single founding NSC. These cautions probably have implications for the use of 'cytospheres' as an assay in other organ systems and with other cell types, both normal and neoplastic. Viscoelastic measurements e. Multiplex assays are used to simultaneously measure the presence, concentration, activity, or quality of multiple analytes in a single test. Sometimes the concentration of the analyte is too large and in that case the assay may involve sample dilution or some sort of signal diminution system which is a negative amplification. Using time-lapse video microscopy, coculture experiments with genetically labeled cells, and analysis of the volume of spheres, we observed that neurospheres are highly motile structures prone to fuse even under ostensibly 'clonal' culture conditions. Like any multi-step information handling and transmission systems, variation and errors in the communicated final results of an assay involve corresponding parts in every such step; i. It might involve a simple centrifugal separation or washing or filtration or capture by some form of selective binding or it may even involve modifying the target e.

These may simply be in the form of a narrow band-pass optical filer, or a blocking reagent in a binding reaction that prevents nonspecific binding or a quenching reagent in a fluorescence detection system that prevents "autofluorescence" of background objects.

These cautions probably have implications for the use of 'cytospheres' as an assay in other organ systems and with other cell types, both normal and neoplastic.

Sometimes the concentration of the analyte is too large and in that case the assay may involve sample dilution or some sort of signal diminution system which is a negative amplification. Generally there are multiple separate steps done before an assay and are called preanalytic processing.

Immunoassay when the response is an antigen antibody binding type reaction. Semi-quantitative assays, i.

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Defining the actual sensitivity and specificity of the neurosphere assay in stem cell biology.